Jiehui Biology: Common pipetting methods

One or two pipetting principles

1. Positive air pressure pipetting

● Air is between the piston and the liquid

● The most commonly used principles

● Recommended:

-Water solution

● Can not eliminate pollutants, need

● Filter tip-safety cone filter

● Need good pipetting technique and experience

● More sensitive to environmental changes (temperature, air pressure)

● Low cost

2. Positive moving pipette

● Contact between liquid and piston

● Completely free from pollution

● Recommended:

-Highly viscous liquid

-Volatile liquids (such as ethanol)

-Foam liquid

● High accuracy

● expensive

Second, how to determine which technology you want to use?

Positive air pressure pipetting technology

1. Positive pipetting (P)

● The most commonly used technology

● Large capacity

● Aqueous solution

● Contains a small amount of detergent or protein liquid

● Solvent (requires pre-flushing)

Positive pipetting steps:

● Press the operation button to the first stop position

● Immerse the tip just below the liquid level (2-3 mm).

● Release the button smoothly and wait for a while.

● Place the button in the second stop position to drain the liquid.

2. Reverse pipetting (rP)

● Absorb actual volume + certain excess liquid

● Biological liquid

● Foam liquid (rich in protein or detergent)

● High viscosity liquid

● Small volume liquid

● High accuracy and precision required

Reverse pipetting steps:

● Put the button in the second stop position

● Immerse the tip just below the liquid level (2-3 mm).

● Release the button smoothly

● Put the button in the first stop position to drain the liquid

● Put the button to the second stop position to discard excess liquid

Beijing Jiehui Biotechnology Co., Ltd. is established on the basis of the State Key Laboratory. It is a high-tech enterprise co-founded by senior personnel with backgrounds in life sciences, biotechnology, and returned overseas students.
The company specializes in operating experimental instruments, research reagents and laboratory consumables in the field of biological sciences, and is committed to providing first-class services to biological science researchers at home and abroad.
At present, the company's products cover biochemistry, molecular biology, immunology, cell biology and other related fields. Due to the characteristics of high quality and stability, high cost performance, etc., it has won a good reputation in the majority of scientific research institutions, research schools and related enterprises. Thus established a three-dimensional marketing network and has a wide range of customer resources.
While providing high-quality products to users, the company always puts customer service and technical support in a more important position than product sales. The company hires full-time and part-time technical support personnel to provide professional and personalized technical services to customers.
Adhering to the concept of "being a person first, then doing things", the company adheres to the tenet of "taking quality as life and customer as center", and dedicate the best quality products and the most considerate service to our customers.

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